Abstract
Overexpression of Sperm Acrosomal SLLP1 Binding protein (SAS1B) in various cancer types, including uterine cancer cells, was discovered a few years ago, and different monoclonal antibodies (anti-SAS1B) that specifically bind to SAS1B antigens were developed. Labeling of these antibodies with radionuclides can provide an opportunity for imaging and radioimmunotherapy. The objective of this study was to label anti-SAS1B (SB5) with Zirconium-89 (89Zr) for PET imaging and determine its biodistribution. Anti-SAS1B (SB5) antibody was labeled with 89Zr indirectly using the chelator desferrioxamine B (DFO), which is currently a best linker for 89Zr. The antibody, SB5, was first conjugated to DFO with a ratio of 1:5 and then labeled with 250 μCi of 89Zr. Results of PET imaging in mouse-bearing uterine cancer tumor showed a limited uptake. The bio-distribution study matched the PET imaging and confirmed the uptake by the tumor, and the accumulation in bones. In conclusion, labeling of anti-SAS1B could provide an effective way of uterine cancer detection and treatment progression.
Highlights
Molecular imaging has had a transformative impact on academic research, industry, and the clinical field [1]
Overexpression of Sperm Acrosomal SLLP1 Binding protein (SAS1B) in various cancer types, including uterine cancer cells, was discovered a few years ago, and different monoclonal antibodies that bind to SAS1B antigens were developed
Labeling of anti-SAS1B could provide an effective way of uterine cancer detection and treatment progression
Summary
Molecular imaging has had a transformative impact on academic research, industry, and the clinical field [1]. Overexpression of SAS1B protein on the cell surface in many types of tumors, including uterine tumor, was discovered by Dr John C, Herr and coworkers, who developed different monoclonal antibodies (anti-SAS1B) against this protein and demonstrated specific binding to SAS1B [11]. Because of their sensitivity and specificity and biological half-live Monoclonal antibodies have been used for more than two decades to target cancer cells [12] [13]. Diagnosis and treatment assessment is crucial for cancer therapy [17]
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