Abstract
In this study, silver nanoparticles were synthesized using Kiwifruit extract. Response surface methodology (RSM) was successfully used to study the effects of AgNO3 concentration and the quantity of Kiwifruit extract on the production of biosynthesized silver nanoparticles. A suitable model between the factors (AgNO3 concentration and the quantity of Kiwifruit extract) and the response (production yield) was statistically developed. Based on the statistical analysis, the first order of extract volume has the most impact on the production yield of silver nanoparticles. Also, the production yield is affected by the second order term of extract volume more significantly than AgNO3 concentration. Further, the interaction between both factors is statistically significant. Under the optimum conditions (AgNO3 concentration >1.9×10−3M and amount of Kiwifruit extract ≈1.0ml); silver nanoparticles in the mean size of 20nm with stability more than 12 months were synthesized. Also, a localized surface plasmon resonance sensor based on silver nanoparticles was developed for determination of H2O2. This sensor has a linear range of 5.0×10−5–5.0×10−3M with a limit of detection value as low as 5.0×10−7M. The applicability of the sensor was demonstrated by analysis of H2O2 in packing sterilant solutions and interference study.
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