Label-free aptamer-based colorimetric detection of mercury ions in aqueous media using unmodified gold nanoparticles as colorimetric probe

Abstract

We report a simple and sensitive aptamer-based colorimetric detection of mercury ions (Hg(2+)) using unmodified gold nanoparticles as colorimetric probe. It is based on the fact that bare gold nanoparticles interact differently with short single-strand DNA and double-stranded DNA. The anti-Hg(2+) aptamer is rich in thymine (T) and readily forms T-Hg(2+)-T configuration in the presence of Hg(2+). By measuring color change or adsorption ratio, the bare gold nanoparticles can effectively differentiate the Hg(2+)-induced conformational change of the aptamer in the presence of a given salt with high concentration. The assay shows a linear response toward Hg(2+) concentration through a five-decade range of 1 x 10(-4) mol L(-1) to 1 x 10(-9) mol L(-1). Even with the naked eye, we could identify micromolar Hg(2+) concentrations within minutes. By using the spectrometric method, the detection limit was improved to the nanomolar range (0.6 nM). The assay shows excellent selectivity for Hg(2+) over other metal cations including K(+), Ba(2+), Ni(2+), Pb(2+), Cu(2+), Cd(2+), Mg(2+), Ca(2+), Zn(2+), Al(3+), and Fe(3+). The major advantages of this Hg(2+) assay are its water-solubility, simplicity, low cost, visual colorimetry, and high sensitivity. This method provides a potentially useful tool for the Hg(2+) detection.