Abstract
A primary advantage of label-free detection methods over fluorescent measurements is its quantitative detection capability, since an absolute measure of material facilitates kinetic characterisation of biomolecular interactions. Mass photometry based on interferometric scattering microscopy (iSCAT) is a novel, label-free imaging and quantification approach, having demonstrated accurate mass measurement of single biomolecules in solution. Here, we show that mass photometry is equally applicable to nucleic acid detection and mass quantification.
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