Abstract
BackgroundLeishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed.ObjectiveThe aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response.MethodsFour patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry.ResultsA total of 12 proteins showed differential expression in the comparative LC-electrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were up-regulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response.ConclusionWe identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in CL.
Highlights
Leishmaniasis is a parasitic disease caused by more than 20 species of Leishmania genus
Based on the advantages offered by this methodology, the aim of this study was to find and validate early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) using proteomics without labeling in order to provide patients with adequate monitoring and identify early treatment failure, providing the possibility of changing the therapeutic scheme in a timely manner
Proteins detected in granulation tissue of CL In total, 344 proteins were identified in the granulation tissue formed in the center of the ulcer
Summary
Leishmaniasis is a parasitic disease caused by more than 20 species of Leishmania genus. Leishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. Failure of treatment is frequent, and the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed. Objective: The aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair These proteins can be useful as biomarkers for early monitoring of therapeutic response in CL
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