Abstract
Southern rice black-streaked dwarf virus (SRBSDV) has spread from thesouth of China to the north of Vietnam in the past few years and severelyinfluenced rice production. Its long incubation period and early symptoms are not evident; thus, controlling it is difficult. Chitosan oligosaccharide (COS) is a green plant immunomodulator. Early studies showed that preventing and controlling SRBSDV have a certain effect and reduce disease infection rate, but its underlying controlling and preventing mechanism is unclear. In this study, label-free proteomics was used to analyze differentially expressed proteins in rice after COS treatment. The results showed that COS can up-regulate the plant defense-related proteins and down-regulate the protein expression levels of SRBSDV. Meanwhile, quantitative real-time PCR test results showed that COS can improve defense gene expression in rice. Moreover, COS can enhance the defense enzymatic activities of peroxidase, superoxide dismutase and catalase through mitogen-activated protein kinase signaling cascade pathway, and enhance the rice disease resistance.
Highlights
Southern rice black dwarf disease is one of the most serious plant diseases worldwide, mainly in East Asia and Southeast Asia, including China, Japan and Vietnam, and results in severe losses in grain production in these areas [1,2]
The results showed that the Chitosan oligosaccharide (COS) treatment groups can significantly improve the content of anthraquinone in Rubia tinctorum L
Label-free quantitative proteomics, enzyme activity test, and RT-qPCR analysis determined the responses of Southern rice black-streaked dwarf virus (SRBSDV)-infected rice to COS
Summary
Southern rice black dwarf disease is one of the most serious plant diseases worldwide, mainly in East Asia and Southeast Asia, including China, Japan and Vietnam, and results in severe losses in grain production in these areas [1,2] Controlling this disease is difficult, because its early symptoms arenot obvious and the viral latency islong. Chitosan oligosaccharide (COS) is an environmentally friendly biological regulator, which can be obtained by hydrolyzing naturally occurring chitosan [4]. It is a type of clean and good biological pesticide source that does not pollute the environment. COS has been considered as a potent elicitor of plant immunity and is used in many plants, such as tobacco [9], wheat [10], camellia
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