Label-Free Quantitative Proteome Analysis Reveals the Underlying Mechanisms of Grain Nuclear Proteins Involved in Wheat Water-Deficit Response.

Tingting Li,Junwei Zhang,Zhisheng Han,Ming Zhang,Dong Zhu,Yueming Yan

doi:10.3389/fpls.2021.748487

Tingting Li, Junwei Zhang + Show 4 more

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https://doi.org/10.3389/fpls.2021.748487

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Abstract

In this study, we performed the first nuclear proteome analysis of wheat developing grains under water deficit by using a label-free based quantitative proteomic approach. In total, we identified 625 unique proteins as differentially accumulated proteins (DAPs), of which 398 DAPs were predicted to be localized in nucleus. Under water deficit, 146 DAPs were up-regulated and mainly involved in the stress response and oxidation-reduction process, while 252 were down-regulated and mainly participated in translation, the cellular amino metabolic process, and the oxidation-reduction process. The cis-acting elements analysis of the key nuclear DAPs encoding genes demonstrated that most of these genes contained the same cis-acting elements in the promoter region, mainly including ABRE involved in abscisic acid response, antioxidant response element, MYB responsive to drought regulation and MYC responsive to early drought. The cis-acting elements related to environmental stress and hormones response were relatively abundant. The transcription expression profiling of the nuclear up-regulated DAPs encoding genes under different organs, developmental stages and abiotic stresses was further detected by RNA-seq and Real-time quantitative polymerase chain reaction, and more than 50% of these genes showed consistency between transcription and translation expression. Finally, we proposed a putative synergistic responsive network of wheat nuclear proteome to water deficit, revealing the underlying mechanisms of wheat grain nuclear proteome in response to water deficit.

Highlights

IntroductionNuclear Proteomic Analysis of Wheat Grains contain a variety of nutrients including starch and storage protein that are rapidly synthesized and accumulated after flowering (Guo et al, 2012; Liu et al, 2012)
As an allohexaploid species and the product of intensive breeding and long-term artificial domestication, wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is the second leading cereal crop widely cultivated in the world
We found that transcription factor TCP20 was significantly up-regulated in wheat developing grains under water-deficit conditions (Supplementary Table 3) and the peak expression level of the TCP20 gene occurred at the grain milk stage (Figure 4A), indicating that TCP20 could play important roles in water-deficit response during wheat grain development

Summary

Introduction

Nuclear Proteomic Analysis of Wheat Grains contain a variety of nutrients including starch and storage protein that are rapidly synthesized and accumulated after flowering (Guo et al, 2012; Liu et al, 2012). Their compositions and properties play important roles in wheat yield and quality formation. Subcellular proteomics has been developed to decipher different organelle proteins involved in abiotic stress defense such as endoplasmic reticulum, plasma membrane, chloroplast, and mitochondria (Wang and Komatsu, 2016; Zhang et al, 2021; Zhu et al, 2021)

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