Abstract
Since changes in the basement membranes are the critical indicators for differentiating normal, precancerous, and cancerous colonic tissues, direct visualization of these warning signs is essential for the early diagnosis and treatment of colonic cancer. Here, we present that second harmonic generation (SHG) microscopy can probe the changes of basement membranes in different colonic cancer stages. Our results also show the capability of using the quantitative analyses of images for quantifying these changes in different cancer stages. These results suggest that SHG microscopy has the potential in label-freely imaging the changes of basement membranes for effectively distinguishing between normal, precancerous, and cancerous colonic tissues. To our knowledge, this is the first demonstration of the dynamics of basement membrane changes in different colonic cancer stages using entirely intrinsic source of contrast.
Highlights
Colonic cancer is one of the major causes of morbidity and mortality in human [1]
This work presents that second harmonic generation (SHG) microscopy can effectively probe the changes of basement membranes in different colonic cancer stages
SHG microscopy enables the identification of two morphologic features, which show significant differences in different colonic cancer stages
Summary
Colonic cancer is one of the major causes of morbidity and mortality in human [1]. It is readily treated if diagnosed in one of the pre-invasive stages [2,3]. Changes in the basement membrane are amongst the most important indicators for colonic cancer development [5,6]. These warning signs have so far only been detectable by histological examination of biopsy specimens [4,7]. The development of new and noninvasive in situ imaging modality for probing these warning signs is of great medical significance
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
