Abstract
A label-free fluorescence aptasensor was studied for adenosine triphosphate (ATP) detection that combines low biosensor toxicity with a simplified preparation process. In this study, the aptamer of the gold nanoparticle@aptamer@carbon quantum dot nanostructure could specifically identify ATP, resulting in the change of the fluorescence signal. In order to analyze the performance of the sensor, the effects of the carbon quantum dot (CQD) concentration and centrifugal rate on the stability of the probe were investigated. The results show that the sensor was superior under the 220 µl CQD volume and 2000 rpm centrifugal rate. Furthermore, the linear relationship between the change of the fluorescence signal and ATP concentration is Y = 359.747 + 0.226X within the volume range of 20 µM–280 µM. The correlation coefficient is 0.98, and the detection limit is 20 µM. No obvious fluorescence change was observed in solutions containing other common ions. On the basis of no pollution and simplicity, this sensor demonstrates great potential as a low-cost diagnostic tool for the detection of various targets, particularly for use in the fields of food safety and biomedical diagnostics.
Highlights
Adenosine triphosphate (ATP) provides a direct source of energy for a broad range of cell metabolisms including muscle contraction, transport of molecules and ions, and synthesis of important biomolecules.1 The adenosine triphosphate (ATP) concentration will sharply drop in the cell when the cell has been dead.2 the abnormal ATP concentration can lead to a number of diseases, such as malignant tumors, hypoglycemia, and Parkinson’s disease.3,4 Current methods to detect ATP include mass spectrometry and high performance liquid chromatography.5,6 these standard technologies generally involve expensive laboratory equipment, highly trained technicians, and timely sample processing.7–9 it is necessary to develop an ATP sensor that is low cost and easy to use
The ATP concentration will sharply drop in the cell when the cell has been dead
The abnormal ATP concentration can lead to a number of diseases, such as malignant tumors, hypoglycemia, and Parkinson’s disease
Summary
Adenosine triphosphate (ATP) provides a direct source of energy for a broad range of cell metabolisms including muscle contraction, transport of molecules and ions, and synthesis of important biomolecules. The ATP concentration will sharply drop in the cell when the cell has been dead. the abnormal ATP concentration can lead to a number of diseases, such as malignant tumors, hypoglycemia, and Parkinson’s disease. Current methods to detect ATP include mass spectrometry and high performance liquid chromatography. these standard technologies generally involve expensive laboratory equipment, highly trained technicians, and timely sample processing. it is necessary to develop an ATP sensor that is low cost and easy to use. Current methods to detect ATP include mass spectrometry and high performance liquid chromatography.. Current methods to detect ATP include mass spectrometry and high performance liquid chromatography.5,6 These standard technologies generally involve expensive laboratory equipment, highly trained technicians, and timely sample processing.. The above sensors can realize the detection of different targets Since they have a certain extent of toxicity and complex marking process, these fluorophores are not beneficial for detecting biomolecules in food.. A label-free fluorescence aptasensor combines the superiority of the aptamer and CQDs to have a broad application in biomolecule and heavy metal ion detection. A label-free fluorescence aptasensor was researched that was conjugated with CQDs for detecting ATP in aqueous solution. The technology of label-free detection provides valuable information for a range of applications including food safety, water analysis, and clinical diagnosis
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