Abstract
The improvement of sensitivity of electrochemical immunosensor can be achieved via two approaches: increasing loading capacities of antibody and enlarging responding electrochemical signals. Based on these, CoFe2O4/graphene nanohybrid (CoFe2O4/rGO) as support was firstly used for preparing electrochemical biosensor, and with the addition of Au@Pd nanorods (NRs) as mimic enzyme, a label-free electrochemical immunosensor was prepared. Due to the high electrical conductivity, open porous structure and large loading capacities of CoFe2O4/rGO, the enhanced signal amplification between Au@Pd NRs and CoFe2O4/rGO was studied. Fabricated as a novel substrate, the prepared immunosensor had a good analytical performance and exhibited a wide linear range from 0.01 to 18.0 ng·mL−1 with a low detection limit of 3.3 pg·mL−1 for estradiol, which was succeeded in applying to detect estradiol in the natural water.
Highlights
Estradiol is a type of environmental estrogens which are chemicals with similar biological activity of estrogens in the body and the environment
With respect to the strategy of electrochemical immunosensor, electrochemical methods based on enzyme or mimic enzyme to realize the signal amplification of antigen and antibody reactions have been mostly studied
To get good analytical performance of label-free electrochemical immunosensor, the capacities for analyte loading and abilities need to be improved for signal amplification
Summary
Estradiol is a type of environmental estrogens which are chemicals with similar biological activity of estrogens in the body and the environment. Various highly sensitive techniques for the detection of estradiol have been extensively developed, such as liquid chromatography (LC), mass spectrometry (MS), gas chromatography (GC), solid-phase extraction-enzyme-linked immunosorbent assay (SPE-ELISA), GC-MS, and LC-MS5–8. Compared with these techniques, electrochemical method has many advantages of ease of operation and low cost[9,10,11,12]. Electrochemical method has many advantages of ease of operation and low cost[9,10,11,12] For this reason, several studies have focused on fabricating electrochemical immunosensor for ultrasensitive and rapid detection of estradiol in recent years[13,14]. Due to the high electrical conductivity, chemical stability, large surface area and open porous structure of these nanohybrids[27,28], they might be used as the substrate for fabricating label-free electrochemical immunosensor
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
