Label free detection of vitamin D by microcantilever-based aptasensor

Abstract

Vitamin D deficiency or insufficiency is a growing public health problem. In this work, a novel and sample approach based on microcantilever for measuring the level of 25-hydroxyvitamin D (25(OH)D) was proposed. The Bruno aptamer was utilized to functionalize the microcantilevers, serving as a sensing layer for detecting 25(OH)D. The working principle of the sensor is based on the changes of the microcantilever deflection and frequency as a result of the aptamer removal from the surface of the microcantilever resulting from its interaction with vitamin D. The results showed a linear dependence between vitamin D concentration and the microcantilever deflection and frequency shift in a range of concentrations from 1 to 300 nM. For this biosensor, the limit of detection (LOD) was calculated to be ∼ 0.3 nM. The biosensor selectivity was tested against vitamin C and the sensor showed high selectivity towards vitamin D.