Abstract
Herein, we report on the fabrication of an extended-gated organic field-effect transistor (OFET)-based immunosensor and its application in the detection of human chromogranin A (hCgA). The fabricated OFET device possesses an extended-gate electrode immobilized with an anti-CgA antibody. The titration results of hCgA showed that the electrical changes in the OFET characteristics corresponded to the glycoprotein recognition ability of the monoclonal antibody (anti-CgA). The observed sensitivity (detection limit: 0.11 µg/mL) and selectivity indicate that the OFET-based immunosensor can be potentially applied to the rapid detection of the glycoprotein concentration without any labeling.
Highlights
Chromogranin A (CgA), which is one of the glycoproteins, exists in secretory granules [1].CgA is processed into small peptides, several of which affect the secretory function of the parent cells [2]
The organic field-effect transistor (OFET)-based immunosensor was composed of two components; the stable operation of the fabricated device was achieved by isolating of the drive unit and the detection portion fabricated device was achieved by isolating of the drive unit and the detection portion
The fabricated OFET can be operated under low voltages (
Summary
Chromogranin A (CgA), which is one of the glycoproteins, exists in secretory granules [1].CgA is processed into small peptides, several of which affect the secretory function of the parent cells [2]. Chromogranin A (CgA), which is one of the glycoproteins, exists in secretory granules [1]. Salivary CgA is known as a marker of psychophysical stress [7,8], such as exposure to situations of anxiety [9,10,11] and depressive mood [12,13]. For these reasons, the monitoring of CgA levels is very important for the management of our health conditions
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