Abstract
Based on the characteristic properties like strong fluorescence and high photostability, DNA-templated silver nanoclusters (DNA-AgNCs) were utilized to detect Klenow fragment exo- (KF-) activity and to monitor its inhibition by daidzein. In this study, fluorescence intensity is linearly correlated for KF- concentration ranging from 0 to 2.0 U/mL, with a minimum detectable concentration of 0.05 U/mL. In addition, KF- inhibition was monitored by changes in fluorescence intensity with increasing daidzein concentration gradually.
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