Abstract
To assess the two dimensional distribution of the extracellular potassium ion concentration ([K+]out) surrounding hippocampal slices, we developed a 128×128pixel type potassium ion-sensitive image sensor (PIS) composed of a charge-coupled-type pH image sensor and a poly(vinyl chloride) (PVC) plasticized membrane containing valinomycin, a potassium ion-sensitive ionophore. The potential slope was 50mV/decade for a potassium ion concentration between 10−3 and 10−1M. Although variations in the signal were not recorded in the absence of a brain slice or in areas outside of the slice position, significant changes in [K+]out were detected underneath the hippocampal slices. We successfully detected the glutamate-induced changes in [K+]out associated with gating through potassium ion channels in hippocampal neurons without a labeling process. [K+]out increased from 10mM to 50mM in the area between the sensor and slice stimulated with glutamate.
Published Version
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