Abstract
A label-free bifunctional electrochemiluminescence (ECL) aptasensor for parallel detection of small molecule (adenosine) and protein (lysozyme) was developed based on switching structures of aptamers from DNA/DNA duplex to DNA/target complex. Thiolated single-stranded DNA (DNA1) containing adenosine aptamer and sequence complementary to DNA2 was first immobilized on the Au electrode via sulfur–gold affinity, DNA2 containing lysozyme aptamer and sequence complememtary to DNA3 was bound to the electrode via forming DNA/DNA duplex. Gold nanoparticles functionalized with DNA3 (DNA3-GNPs) were used as signal amplifier to enhance the sensitivity of the aptasensor. With Ru(phen)32+ as the signal transducer, the aptasensor exhibited high sensitivity and specificity. Under optimal conditions, a linear range from 0.5 to 7nM for adenosine (R=0.999) and from 0.5 to 9ng/mL for lysozyme (R=0.999) was obtained, respectively. The proposed method may provide a promising platform for ECL parallel detection of small molecules and protein.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
