Abstract
We develop a simple and label-free fluorescence method for the polynucleotide kinase (PNK) assay based on phosphorylation-triggered isothermal exponential amplification. This method exhibits ultrahigh sensitivity superior to most existing PNK assays. Importantly, this method enables sensitive detection of endogenous PNK activity at the single-cell level, holding great potential in clinical diagnosis and biomedical research.
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