Abstract
The process of unwinding and renaturation of DNA has been widely used in studies of nucleotide sequence organization. Compared with traditional methods for DNA unwinding and renaturation, the label-free and non-destruction detection technology is significant and desiderated. We realized an optical system based on optical rotation via weak measurement for detection of single- and double-strand state of DNA. The optical rotation, which was induced by the status change of single and double DNA strands, was exploited to modulate the preselected polarization of a weak measurement system. With this modulation, the optical rotation caused by the separation of DNA strands can be determined through the center wavelength shift of the output spectrum. By monitoring the wavelength shift in real time, the separation processes of the DNAs with different base ratio (25% and 70%) and length (4nt and 40nt), and DNAs with three terminally modified cholesterol molecules were experimentally explored in varied pH and temperature conditions. In addition, the detection limit of the DNA concentration was obtained to be 5 × 10−6 mol/L. Our work based on optical rotation detection of single- and double-strand DNA exhibits the unique advantages of real-time monitoring, label-free, non-destruction and simplicity.
Highlights
We demonstrate the effect of the weak measurement system by optical rotation response experiment
We conducted some studies on the real-time non-destructive detection of ssDNA and dsDNA states using weak measurements
When the DNA changes from the dsDNA state to the ssDNA state, the center wavelength shifts toward the decreasing direction
Summary
Tian Guan[1,2], Yuxuan Yang[1,2], Qianwen Zhang[4], Yonghong He1,2, Naihan Xu4, Dongmei Li2,3, Lixuan Shi[2,3], Yang Xu2,3 & Xiangnan Wang[1,2]. A transmission-based optical rotation detection method based on weak measurement is proposed, which can be used to amplify weak signals, and has been introduced into many high-precision measurements[12,13,14,15,16,17] In this system, the pre-selected polarization state is changed using the optical activity of the DNA liquid during the change of the single- and double-strand states. External real-time monitoring and lossless labeling have no effect on DNA solutions This weak measurement scheme provides a new method for the study of DNA molecule denaturation, with advantages of high accuracy, robustness, simple operation, and fast response. The final state of the system can be expressed as: ψfinal
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