Abstract
This study was aimed to obtain the considerable amount of the biosurfactant (rhamnolipid) from local isolate of Pseudomonas aeruginosa on industrial-scale. A 7L laboratory bioreactor was appointed to accomplish this task and all conditions of production were set as previously by batch culture experiments. Bioreactor with 3.5 L working volume was conducted for 120 h to obtain 16 g/L at 30oC and 6.5 pH. The supernatant of production medium was used to reduce the surface tension of water from 72 to 27 mN/m, and the emulsification index was reached to 67%. The product was extracted with a mixture of solvents, purified by silica gel using a column of glass chromatography (3.5 × 30cm), and characterized by TLC and FTIR where it turn out that the biosurfactant is composed of both mono- and di-rhamnolipid. Mono-RL was tested for its antibacterial activity against some clinical isolates of Gram-positive and Gram-negative bacteria to obtain the MIC, which was about 250µg/ml, also at same concentration, the toxicity against Rat embryonic fibroblast (REF) cell line was reached to 23%. The combination effect of Mono-RL with some antibiotics was studied against pathogenic clinical isolates revealed that there is a synergistic effect with ampicillin against S. aureus and Serratia marcescens, with cefotaxime against E. coli and Kelbsiella pneumoniae, same as with tetracycline.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.