Abstract

An acetonic powder of the kidney of rabbit incubated at pH 7§d3, under nitrogen, at 3°, in the presence of sulphur-labelled sulphite, pyruvic acid and glutamic acid leads to the enzymic formation of a labelled substance, which is shown by paper chromatography using various solvents and by paper ionophoresis at various pH's to behave like cysteinesulphinic acid. The performic oxidation of this substance yields a compount shown by paper chromatography to be identical to cystenic acid. The condensation of sulphite on an organic molecule has certain analogies with the fixation of CO 2 by pyruvic acid, but its mechanism has not yet been elucidated.

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