La culture in vitro de conifères alpins

Abstract

Abstract The in vitro culture of alpine conifers. Seeds of European larch, Scots pine, Mugo pine and Norway spruce were sterilized for 30 min in a bath of 30% hydrogen peroxide.Similarly, seeds of Swiss stone pine, of which the tegument had been removed with a little vice, were sterilized for 15 min.Germination and a sterility test were undertaken on malt‐agar. Finally, the seeds were transplanted in vitro on two media for mycorrhizal synthesis.