L1 Gene based Molecular Characterization of Bovine Papillomavirus Type 1 (BPV1) Isolated from Cutaneous Warts of Cattle, Maharashtra

Abstract

Background: Bovine papillomatosis (BP) is distributed worldwide among cattle but is relatively less common in buffaloes. Prevalence of this disease is established in buffaloes from India and Italy caused by bovine papilloma virus (BPV). The current study aimed to study the detection of bovine papilloma virus from cutaneous warts by polymerase chain reaction and confirmed by nucleotide sequencing and phylogenetic analysis. Methods: In this study a total of 10 wart samples from affected cattle were aseptically collected. Grossly, most of cutaneous warts were of variable sizes, rough or coarse in texture, grayish/blackish/flesh colored, irregular in shape (dome or button) or resembling cauliflower-like masses and elevated from skin surface by broad base. Molecular characterization of viral DNA from wart samples and partial gene sequencing, its analysis were carried out by using bioinformatics tools to establish the phylogenetic relationship. Result: In our investigation it revealed that out of total 10 cutaneous wart tissue samples, only six cattle were positive for BPV-1 while all the samples were found negative for BPV-2. The phylogenetic analysis of BPV-1gene of papilloma virus of cattle showed 100% homology with already reported sequence from china. The BPV-1 partial sequences generated in present study revealed close homology as well as with the earlier published BPV-1 sequences on NCBI.