Abstract
Abstract The accumulation of L -threonine by the methylotrophic bacterium Methylobacillus flagellatum KT occurs via a specific system that is capable of transporting L -threonine against a 100-fold concentration gradient. This transport system demonstrates the following kinetic parameters: K m = 0.2 mM and V max = 2.5 nmol/min/mg of cells (dry weight). The activity of the system is inhibited by oxidative phosphorylation uncouplers and valinomycin. Cytoplasmic L -threonine does not leak from the cell, but bacteria are capable of exchanging exogenous L -threonine for its intracellular counterpart.
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