Abstract
A glycine-resistant mutant, GM2, showing improved l-serine production was derived from Hyphomicrobium methylovorum KM 146. The mutant was found to have elevated methanol dehydrogenase and serine transhydroxymethylase activities. Using the mutant GM2, the optimum reaction conditions for serine production by resting cells were determined. The optimum concentrations of glycine, methanol and cells were 100 mg/ml, 48 mg/ml and 30 mg (as dry matter)/ml, respectively. The optimum buffer was 0.05 to 0.08 m Tris-HCl buffer, pH 10.2. Appropriate aeration was necessary. Methanol feeding and glycine feeding improved the l-serine production. Under the optimum conditions, 32 ~ 34 mg/ml of l-serine was produced.
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