L-Proline adsorbed oxygen-loaded nanobubbles in-situ gel for wound healing

Abstract

AimWound healing is a natural physiological process that restores the damaged layers of the skin by the migration and proliferation of fibroblasts. This present research work aimed to develop a nanoformulation gel of oxygen and L-proline to offer a synergistic effect on the wound healing process for better patient compliance and protection from cross-infections. Main methodsThe nanobubbles were prepared using the gas entrapment method, followed by cold addition for the synthesis of in-situ gel and further characterized by different parameters like dissolved oxygen level, particle size and zeta potential, in-vitro, ex-vivo release and animal studies. Key findingsL-Proline oxygen nanobubbles in-situ gel formulation was prepared by incorporating oxygen concentration of 15.5 mg/L at pH 5.6. The concentration of dissolved oxygen was stabilized for 6 h and displayed synergistic effects with L-proline at the sol-gel transition temperature. In-vivo studies demonstrated complete wound healing in 21 days and 27 days for oxygen nanobubble with L-proline and control group, respectively. SignificanceThis controlled release nanoformulation showed a combinational effect of oxygen and L-proline for reducing the frequency of application compared to the marketed formulation.