Abstract
l-Pipecolate oxidase, an enzyme that oxidizes l-pipecolic acid in the human liver has been demonstrated in the peroxisomal preparation. This enzyme oxidizes l-pipecolic acid with concomitant production of H 2O 2 in the peroxisome of the normal human liver. The immediate product of l-pipecolic acid oxidation has been identified as l-α-aminoadipate δ-semialdehyde. This reaction product was directly, and also after conversion to pipecolic acid by NaBH 4 reduction, characterized by use of an amino acid analyzer and thin-layer chromatography. The pit fall of an indirect assay of l-pipecolate oxidase by means of the assay of α-aminoadipic acid formation was discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
