L-Malic acid production from n-paraffins by candida brumptii IFO-0731.

Abstract

Candida brumptii IFO-0731, which is known to produce succinic acid from n-paraffins, also produced other organic acids. One of them was identified as L-malic acid. Some culture conditions for L-malic acid production from n-paraffin were investigated. It was found that urea was the most favorable nitrogen source and 2g/liter of urea was optimum for L-malic acid production. Under the best condition, 25.4g/liter of L-malic acid was obtained from 4% (v/v) of super heavy n-paraffin and the yield was about 80% (wt./wt.).