L-Lactate Transport and Metabolism in Mitochondria of Hep G2 Cells-The Cori Cycle Revisited.

Salvatore Passarella,Avital Schurr

doi:10.3389/fonc.2018.00120

Abstract

l-Lactate Transport and Metabolism in Mitochondria of Hep G2 Cells-The Cori Cycle Revisited.

Highlights

Specialty section: This article was submitted to Molecular and Cellular Oncology, a section of the journal Frontiers in Oncology
In the 1920s, Warburg found that cancer cells prefer to produce ATP by glycolysis with l-lactate production, to the best of our knowledge, the mitochondrial metabolism of l-lactate had not been investigated in cancer cells until 2010, when the first evidence for l-lactate mitochondrial metabolism in these cells was published [18]
Cori Cycle in Cancer Cells intramitochondrial pyridine nucleotides, as shown by fluorimetric measurements, upon the addition of l-lactate to mitochondria indicates that l-lactate metabolism occurs inside the organelles via an NAD+-dependent mitochondrial l-lactate dehydrogenase (m-l-LDH); the occurrence of the mitochondrial l-lactate metabolism in cancer cells was not quoted in Ferguson et al [17] possibly because the authors of the review consider the spectroscopic and polarographic techniques to be “problematic,” despite its widespread use by numerous scientists

Summary

Introduction

Specialty section: This article was submitted to Molecular and Cellular Oncology, a section of the journal Frontiers in Oncology. To study l-lactate transport and metabolism in mitochondria isolated from both normal and cancer prostate cells, spectroscopic and polarographic techniques were used, in which either m-l-LDH reaction or oxygen consumption by mitochondria, supplied with externally added l-lactate were monitored, respectively [19], rather than employing more involved procedures, available in molecular biology, genetics, and chemistry laboratories.

Results

Conclusion