L'idiotypie comparée des IgG et des IgM de lapin anti- Salmonella abortus-equi

Abstract

We have compared idiotypic specificities of rabbit anti- Salmonella abortus-equi (S.a.e.) antibodies which were IgG or had the behaviour of macroglobulins (Mg) during immunoelectrophoresis and exclusion chromatography. These IgG and Mg fractions were prepared from two different anti-S.a.e. serum samples collected from a rabbit after two series of injections of bacteria made at an interval of three weeks. These IgG and these Mg were respectively used for the preparation of anti-idiotypic sera. Some anti-S.a.e. IgG can bear idiotypic specificities which are not detected on anti-S.a.e. Mg, and some anti-S.a.e. IgG can bear idiotypic specificities which are also detected on anti-S.a.e. Mg. These two distinct IgG idiotypes have apparently the same isotypic specificities. These isotypic are different from those borne by other serum IgG which consequently must belong to another subclass. Idiotypic patterns borne by one Mg molecule can be distributed on several IgG molecules. We have observed only one case where idiotypic patterns which seemed to be borne by one Mg molecule in the serum of a bleeding were distributed on two Mg molecule in the serum of the following bleeding.