L'idiotypie comparée des anticorps du sérum, des anticorps du lait, des chaînes polypeptidiques d'IgG et des chaînes polypeptidiques D'IgM de lapins immunisés contre Salmonella abortus-equi

Abstract

Antibodies against Salmonella abortus-equi (S.a.e.) and idiotypic specificities carried by serum anti-S.a.e. antibodies are detected in milk immunoglobulins and in new-bron rabbit serum. Anti-idiotypic sera from rabbits submitted to injections of bacteria agglutinated by serum antibodies did not distinguish between milk IgG and serum IgG. They also did not distinguish milk IgM from serum IgM. The fraction containing milk IgA faintly precipitated Salmonella abortus-equi somatic antigen; it was not precipitated by anti-idiotypic sera. The heavy chains of IgG were precipatated (rather weakly) by 13 anti-idiotypic sera out of 13. Light chains of IgG were only precipitated by anti-idiotypic sera obtained after longer immunization. It appears from a combination of precipitation reactions in liquid medium and in gel medium that γ chains and μ chains are involved in idiotypic specificities common to IgG and IgM.