L-Glutaminase production by marine Beauveria sp. under solid state fermentation

Abstract

Extracellular l-glutaminase production by Beauveria sp., isolated from marine sediment, was observed during solid state fermentation using polystyrene as an inert support. Maximal enzyme production (49.89 U/ml) occurred at pH 9.0, 27°C, in a seawater based medium supplemented with l-glutamine (0.25% w/v) as substrate and d-glucose (0.5% w/v) as additional carbon source, after 96 h of incubation. Enzyme production was growth associated. Results indicate scope for production of salt tolerant l-glutaminase using this marine fungus.