Abstract
L- asparaginase ( EC 3.5.1.1) is an enzyme which converts L- asparagine to L-aspartic acid and ammonia; L- glutaminase ( EC 3.5.1.2) is an enzyme which catalyzes the conversion of L-glutamine to L- glutamic acid and ammonia. Both of these enzymes have been used in as chemotherapeutic agents. Among different sources of L- asparaginase and Lglutaminase enzymes producers, microbial strains possess an elevated edge over other enzyme producers; these enzymes produced by different microbial strains differed in some physiological, biochemical, catalytic and immunological properties. This led to the continuous screening program for isolation of novel microbial strains that could produce an effective enzyme with few limitations at use. A bacterial strain isolated from soil and identified as Psedomonas aeruginosa had been found to be capable of producing both extracellular L- Asparaginase and L- Glutaminase enzymes. The enzymes were produced under solid state fermentation. Effects different fermentation parameters for production of these enzymes were determined. Some physicochemical properties of both of these enzymes were determined. The results obtained in this study revealed the potential of Pseudomonas aeruginosa as a source of both L-Asparaginase and L- Glutaminase enzymes, which have gained significance in pharmaceutical industry. The uses of inexpensive agro- industrial wastes in this study have important economic advantages over submerged fermentation.
Highlights
L- asparaginase ( EC 3.5.1.1) is an enzyme which converts L- asparagine to Laspartic acid and ammonia has been used as a chemotherapeutic agent
This led to the continuous screening programme for isolation of novel microbial strains as effective producers of these enzymes with few limitations.In recent times, the bacterial systems are increasingly investigated for the production of enzymes and metabolites by solid-state fermentation (SSF) .The SSF has numerous advantages over submerged fermentation (SmF), including superior productivity, simple technique, low capital investment, low energy requirement and less water output,better product recovery and lack of foam build-up (Carrizales and Jaffe, 1986).the utilization of agrowastes as a substrate for carbon and energy requirement under SSF makes this approach environment friendly
In this paper we report the production of both extracellular L-asparaginase and Lglutaminase enzymes by an isolated strain of Pseudomonas aeruginosa PAO1 under SSF using Wheat Bran
Summary
L- asparaginase ( EC 3.5.1.1) is an enzyme which converts L- asparagine to Laspartic acid and ammonia has been used as a chemotherapeutic agent. The literature reports suggested that these enzymes produced by different microorganisms differed in some physiological, biochemical, catalytic and immunological properties (Jeya Prakash et al, 2009; Mishra, 2006, ElBessoumy et al, 2004). This led to the continuous screening programme for isolation of novel microbial strains as effective producers of these enzymes with few limitations.In recent times, the bacterial systems are increasingly investigated for the production of enzymes and metabolites by solid-state fermentation (SSF) .The SSF has numerous advantages over submerged fermentation (SmF), including superior productivity, simple technique, low capital investment, low energy requirement and less water output ,better product recovery and lack of foam build-up (Carrizales and Jaffe, 1986).the utilization of agrowastes as a substrate for carbon and energy requirement under SSF makes this approach environment friendly. Attempts were made to study the optimization of both L-asparaginase and L-glutaminase production and preliminary characterization of both of the crude enzymes
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