L-Arginine-selective microbial amperometric sensor based on recombinant yeast cells over-producing human liver arginase I

Abstract

The article provides a description of a microbial l-arginine-selective amperometric biosensor based on recombinant cells of the methylotrophic yeast Hansenula polymorpha. The intact and permeabilized cells (p-cells) co-immobilized with commercial urease on a surface of electrode were used as biorecognizing elements of biosensors. Operational parameters of the bioelectrodes were estimated. The developed p-cells-based sensor revealed a satisfactory selectivity to l-arginine (Arg) and sensitivity (14±1.2A(Mm2)−1) with an apparent Michaelis–Menten constant derived from Arg calibration curve 0.51±0.05mM. The dynamic range is linear till 0.6mM Arg, a detection limit is 0.085mM and a response time 60s. The developed microbial biosensor was used for Arg assay in real samples of wine and juice in comparison with other analytical approaches. The Arg values obtained with different methods demonstrated a strong correlation.