Abstract
Possible effects of tetanus toxin (TeTox) on voltage-activated Ca 2+ channels of the mouse neuroblastoma cell line N1E-115 and of the neuroblastoma x glioma hybrid cell line NG108-15 have been investigated using the whole-cell voltage clamp technique. Similar to N1E-115 cells, differentiated NG108-15 cells express transient (T-type) as well as long-lasting (L-tyoe) Ca 2+ channels. Using various treatment protocols N1E-115 and NG108-15 cells were exposed to TeTox externally and by internal dialysis. In the cells treated with TeTox normal Ca 2+ channel activity was present, as measured by the voltage-activated Ba 2+ currents under voltage clamp conditions. In addition, intracellular microelectrode recordings showed that TeTox did not block the Ca 2+ action potential in N1E-115 cells. It is concluded that TeTox, in contrast to previously reported results 20,21, does not affect voltage-activated T- and L-type Ca 2+ channels in cultured neuronal cell lines. The results also indicate that Ca 2+ channel block is unlikely to be an explanation for the block of neurotransmitter release by TeTox in vivo.
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