Abstract
Erythrina plants known plants “dadap” is a higher plant that grows in tropical and subtropical regions. E. poeppigiana plants was a source of secondary metabolites, which contain flavonoids. This study aims to isolate the flavonoid compounds from the leaves of E. poeppigiana through the stages of extraction, fraction, separation and purification. E. poeppigiana leaves powder (2.5 kg) was extracted with methanol and partitioned with n-hexane and ethyl acetate. Furthermore, the separation of ethyl acetate of E. poeppigiana leaves fraction using a combination of column chromatographic was obtained pure compound (5 mg) in the form of a yellow amorphous solid. The chemical structure of pure compound was based on the data spectroscopy (MS, UV, IR, 1H-NMR and 13C-NMR) and identified as the compound 3,3 ‘, 4’, 5,7-pentahidroksiflavon or known as quercetin.
Highlights
Erythrina plants known plants “dadap” is a higher plant that grows in tropical and subtropical regions
This study aims to isolate the flavonoid compounds from the leaves of E. poeppigiana through the stages of extraction, fractionation, separation and purification
Tanaman Obat Indonesia. http:// www.dephut. go.id/indexphp? =id /node/54 (06 Mei 2016)
Summary
Bahan tumbuhan yang digunakan dalam penelitian ini adalah bagian daun tumbuhan E. poeppigiana yang dikumpulkan dari Subang, Jawa Barat pada bulan Maret 2016 dan telah dideterminasi di Laboratorium Taksonomi Departemen Biologi UNPAD. Fraksi etil asetat (3,5 g) dipisahkan menggunakan metode kromatografi kolom dengan pelarut nheksana:aseton (7:3 sampai 4:6) secara gradien, diperoleh delapan fraksi gabungan (F1-8). Fraksi F3 menunjukkan noda yang berpendar pada UV λ254 nm dan berpendar pada λ365 nm yang merupakan ciri dari senyawa flavonoid. Fraksi F3 (0,1778 g) dipisahkan dengan menggunakan metode kromatografi kolom menggunakan fasa diam silika gel G60 (70-230 mesh) yang dielusi dengan pelarut isokratik klorofom:etil asetat (9:1), diperoleh enam fraksi gabungan yaitu F3A-F. Untuk menguji kemurnian dari isolat F3D dilakukan teknik kromatografi lapis tipis pada pelat ODS yang dielusi dengan pelarut metanol:air (1:1) dan pada pelat silika menggunakan pelarut nheksana:etil asetat (1:1) dan pelarut metilen klorida:etil asetat (7:3), yang masing-masing menunjukkan noda tunggal
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