Abstract
Artificial insemination (AI) is a technique to insert the sperm or semen of bull thawed and processed first into the genital tract of female methods and a special tool called insemination gun, efforts to optimize the management of the cement in order to obtain the quality of cement is optimal to do with the selection of diluent cement. One alternative materials cement diluent is an isotonic solution Commercial (LIK). This study aimed to examine the interaction between the concentration and duration of storage of diluent solution isotonic different to the quality of spermatozoa cow simental. This research was conducted at the Regional Technical Implementation Unit of Artificial Insemination Centres (UPTD BIB) Tuah Sakato, Payakumbuh. In April 2016. This study used a randomized block design (RAK) factorial design to Factor A (Concentration LIK) ie A1 = 100 ml Tris Egg Yolk, A2 = (60 ml) Tris Egg Yolk + (40 ml) LIK, A3 = (55 ml) Tris Egg Yolk + (45 ml) LIK, A4 = (50 ml) Tris Egg Yolk + (50 ml) LIK, and factor B (retention) is B1 = 0 day, B2 = 1, B3 = 2 day, days, B4 = 3 days. Each treatment consists of three replicates. Parameters measured were motility, percentage of survival, abrnormalitas, and MPU. These results indicate that an isotonic solution can be used as an alternative Commercial diluent with the best results on the percentage LIK 40 ml, but the effectiveness of the efficiency of storage use was only on the first day (24 hours).
Highlights
Kemajuan bioteknologi peternakan dewasa ini diarahkan dalam bidang reproduksi salah satunya adalah Inseminasi Buatan (IB)
This study aimed to examine the interaction between the concentration and duration of storage of diluent solution isotonic different to the quality of spermatozoa bull simental
These results indicate that an isotonic solution can be used as an alternative Commercial diluent with the best results on the percentage LIK 40 ml, but the effectiveness of the efficiency of storage use was only on the first day (24 hours)
Summary
Salah satu minuman yang mengandung asam sitrat adalah Pocari Sweat, dalam penelitian ini akan disebut Larutan Isotonis Komersial (LIK). Berdasarkan penelitian Ichmy (2010), didapatkan pH LIK Kuning Telur (LIKKT) sebesar 6,3 (pH LIKKT hampir mendekati pH semen sapi simental yaitu 5,9–7,3 (Susilawati dkk., 1993). Salah satu unsur yang terkandung dalam LIK adalah citrate 0 mEq/L (Pocari Sweat, 2015) yang artinya sama dengan 1,92% dalam 100 ml sedangkan jumlah citrate yang digunakan dalam perlakuan kontrol sebanyak 2,9%. Pada penelitian Ichmy (2010) penggunaan bahan pengencer 1:4 (LIK+Kuning Telur) hasilnya tidak memberikan pengaruh nyata terhadap motilitas spermatozoa domba, tetapi pada perlakuan bahan pengencer 1:2:2 (kuning telur + LIK + tris) persentase motilitas yang didapat adalah 51%, sedangkan tris kuning telur persentase motilitas yang didapat adalah 51,1%, dimana kedua bahan pengencer tersebut tidak berbeda nyata
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have