Kronik İmmobilizasyon Stresine Maruz Bırakılan Ratlarda Fluoksetin ve Gundelia tournefortii L. Bitki Ekstresinin; Anksiyete, Motor Aktivite, Böbrek ve Karaciğer Dokuları Üzerine Etkisinin Belirlenmesi

Abstract

This study was completed with the aim of investigating the effects of extracts obtained from Gundelia tournefortii L. and fluoxetine on anxiety, motor activity, biochemical and antioxidant parameters in rats exposed to chronic immobilization stress. The study included a total of 40 female Wistar albino rats with live weight of 200-220 g, randomly divided into groups of 8 rats with the study duration planned as 30 days. The groups created in the study were “control”, “physiologic serum + chronic immobilization”, “Gundelia tournefortii L. plant extract”, “Gundelia tournefortii L. plant extract + chronic immobilization” and “fluoxetine + chronic immobilization”. At the end of 30 days, all the groups had rotarod test (motor activity) and anxiety test (elevated plus maze) applied and after all the tests were completed the rats were sacrificed. At the end of the study, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), uric acid (UA), creatinine (CRE), total protein (TP) and albumin levels were determined. Additionally, catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione S-transferase (GST), glutathione reductase (GR) activity and reduced glutathione (GSH) and malondialdehyde (MDA) levels were identified in liver and kidney tissue samples. In conclusion, administration of both Gundelia tournefortii L. plant extract and fluoxetine can be said to have positive effects on enzyme activities and MDA parameter value against experimentally-induced immobilization stress. Additionally, Gundelia tournefortii L. was not identified to have any effect on motor activity, but identified to have anxiolytic effect according to elevated plus maze test results.