Abstract
A beige pigmented bacterial strain (JM-387T), isolated from field-grown corn root tissue, Tallassee, Alabama, was studied for its taxonomic allocation. A comparison of the 16S rRNA gene sequence with those of the type strains of most closely related species of the family Enterobacteriaceae showed highest sequence similarities to the type strain of Kosakonia sacchari (99.5%), “Enterobacter oryzendophyticus” (98.8%), and Kosakonia radicincitans (98.6%).Construction of phylogenetic trees based on the 16S rRNA gene and partial sequences of four protein-coding genes, rpoB, gyrB, infB, and atpD (multilocus sequence analysis, MLSA) showed a distinct clustering of strain JM-387T with Kosakonia sacchari. DNA-DNA hybridizations between JM-387T and the type strains of most similar Kosakonia/“Enterobacter” species including K. sacchari LMG 26783T, “E. oryzendophyticus” LMG 26432T, K. radicincitans D5/23T, K. oryzae LMG 24251T, E. cancerogenus LMG 2693T, and E. cloacae subsp. dissolvens CCUG 25230T were in the range of 14.4–60.2%. The average nucleotide identity (ANI) of the genome sequence of the new strain to K. sacchari SP1T was 94.47%.Strain JM-387T had a typical enterobacterial fatty acid pattern consisting of the major fatty acids C16:0, C16:1 ω7c/C16:1 ω6c/C15:0 2OH, C18:1 ω7c/C18:1 ω6c with C14:0 3-OH as hydroxylated fatty acid.Genotypic data and the differentiating biochemical and chemotaxonomic properties showed that strain JM-387T represents a novel species of the genus Kosakonia, for which the name Kosakonia pseudosacchari sp. nov. (type strain JM-387T=CIP 110597T=DSM 27151T) is proposed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
