Abstract
Sapodilla (Achras zapota L.) is a plant commonly found on the island of Java, West Sumatra and West Nusa Tenggara. Sapodilla plant contains flavonoids, saponins and tannins. In leaf the dominant are flavonoids. The content of flavonoids in the plant tissue may act as an antioxidant and antibacterial. As an alternative natural antibacterial, sapodilla leaves should also be tested minimum inhibitory concentration effective to inhibit the bacteria Staphylococcus aureus. This study aims to determine the minimum inhibitory concentration sapodilla leaf extract (Achras zapota L.) on the growth of Staphylococcus aureus. The research was conducted from January to February 2015 in the Laboratory of Microbiology UNP. Research method is descriptive by observing turbidity levels at each test tube as well as comparison increment Optical Density (OD) before incubation and after incubation of zeroes. Sapodilla leaf extract concentration ranging from 10% - 50%, amoxilin as a positive control, and negative controls (without extract). Study results showed that the minimum inhibitory concentration of Sapodilla leaf extract on the growthbacteria present in a concentration of 50%. Keywords : Sapodilla leaf extract , Minimun Inhibitory Concentration (MIC), and Staphylococcus aureus
Highlights
This study aims to determine the minimum inhibitory concentration sapodilla leaf extract (Achras zapota L.) on the growth of Staphylococcus aureus
Hasil penelitian dan pembahasan yang telah dilakukan dapat disimpulkan bahwa nilai konsentrasi hambat minimum ekstrak daun sawo (Achras zapota L.) didapat pada konsentrasi 50%
Summary
Januari sampai bulan Februari 2015 di Laboratorium Mikrobiologi dan Laboratorium Fisiologi Tumbuhan Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang. Pembuatan seri konsentrasi ekstrak Ekstrak dari daun sawo dibuat 8 seri konsentrasi mulai dari 0%, 10 %, 20 %, 30%, 40%, 50%, dan 10 mL amoxicillin 500 mg yang dimasukkan ke dalam 7 tabung reaksi steril yaitu a. 2. Pengujian daya antibakteri Setiap tabung percobaan diisi dengan 8 mL medium Nutrient Broth ditambahkan 0,5 mL ekstrak mulai dari konsentrasi 10% - 50% pada masing- masing tabung reaksi lalu ditambah 0,5 mL suspensi bakteri uji (Staphylococcus aureus). Lalu diambil 3 mL tiap tabung reaksi secara aseptis untuk diukur absorbansinya (Optical Density= OD) pada spektrofotometer (λ = 480 nm) .Kemudian tabung reaksi ditutup dengan kapas steril, alumunium foil dan plastik warp steril. Pengamatan dilakukan dengan melihat kekeruhan pada tiap tabung reaksi dan mengukur nilai OD dengan spektrofotometer
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