Abstract
Potato virus Y (PVY) and Phytophthora infestans (Mont.) de Bary that causes potato late blight (LB), pose serious constraints to cultivated potatoes due to significant yield reduction, and phenotyping for resistance remains challenging. Breeding operations for vegetatively propagated crops can lead to genotype mislabeling that, in turn, reduces genetic gains. Low-density and low-cost molecular marker assessment for phenotype prediction and quality control is a viable option for breeding programs. Here, we report on the development of kompetitive allele specific PCR (KASP) markers for LB and PVY resistance, and for routine quality control assessment of different breeding populations. Two KASP markers for LB resistance and two for PVY Ryadg were validated with an estimated assay power that ranged between 0.65 and 0.88. The developed QC KASP markers demonstrated the capability of discriminating tetraploid calls in breeding materials, including full-sibs and half-sibs. Routine implementation of the developed markers in a breeding program would assist with better allocation of resources and enable precise characterization of breeding material, thereby leading to increased genetic gains.
Highlights
IntroductionThe oomycete Phytophthora infestans (Mont.) de Bary, which causes potato late blight (LB), and potato virus Y (PVY), a member of the genus Potyvirus, are two of the most important potato pathogens worldwide and, if not controlled, can damage entire plants and cause yield reductions of up to 80% [4,5,6]
Propagated, cultivated potato (Solanum tuberosum L.) is an autotetraploid crop (2n = 4x = 48), which leads to high levels of genetic heterogeneity and increases the complexity of genomic studies and breeding [1]
9 single nucleotide polymorphism (SNP) or indels were selected for kompetitive allele specific PCR (KASP) marker design, and the amplification results were compared with a high-resolution melting (HRM) assay using amplification results were compared with a high-resolution melting (HRM) assay using probe M6P2 [17] on a quality evaluation set of 72 tetraploid potato genotypes from the probe M6P2 [17] on a quality evaluation set of 72 tetraploid potato genotypes from the CIP breeding program
Summary
The oomycete Phytophthora infestans (Mont.) de Bary, which causes potato late blight (LB), and potato virus Y (PVY), a member of the genus Potyvirus, are two of the most important potato pathogens worldwide and, if not controlled, can damage entire plants and cause yield reductions of up to 80% [4,5,6] The incidence of these two diseases has increased due to several factors, including climate variations, inadequate crop management, the strain and pressure of the pathogen, and the use of nonresistant varieties [7,8,9]. The varietal resistance approach is of utmost importance for smallholder farmers and the environment, given the lack of information on the pathogen, the stringent crop management requirements (e.g., clean seeds and crop rotation), and the cost and unsustainable nature of chemical control [12,13,14]
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