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Abstract
Kojic acid (5-hydroxy-2-(hydroxymethyl)-4H-pyran-4-one; also named 5-hydroxy-2-(hydroxymethyl)-γ-pyrone) in the presence of hydrogen peroxide, but not in its absence, can be oxidized by hemoglobin (Hb) to a yellow product(s). The yellow product(s) formed is characterized by a peak at 370-380 nm and is fluorescent. The relationship between the rate of oxidation of kojic acid and various concentrations of hemoglobin and of H 2 O 2 is described. The changes with time in the spectrum of product(s) obtained when kojic acid is acted upon by the Hb/H 2 O 2 system and the relationship between the various concentrations of hemoglobin, of kojic acid and of H 2 O 2 on the spectrum of the final yellow product(s) are shown.
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