Abstract
The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain. Unbiased stereology, the method accepted as state-of-the-art for quantification of biological objects in tissue sections2, generates reliable structural data for biological features in the mammalian brain3. The key components of the approach are unbiased (systematic-random) sampling of anatomically defined structures (reference spaces), combined with quantification of cell numbers and size, fiber and capillary lengths, surface areas, regional volumes and spatial distributions of biological objects within the reference space4. Among the advantages of these stereological approaches over previous methods is the avoidance of all known sources of systematic (non-random) error arising from faulty assumptions and non-verifiable models. This study documents a biological application of computerized stereology to estimate the total neuronal population in the frontal cortex of the vervet monkey brain (Chlorocebus aethiops sabeus), with assistance from two commercially available stereology programs, BioQuant Life Sciences and Stereologer (Figure 1). In addition to contrast and comparison of results from both the BioQuant and Stereologer systems, this study provides a detailed protocol for the Stereologer system.
Highlights
The non-human primate is an important translational species for understanding the normal function and disease processes of the human brain
The method accepted as state-of-the-art for quantification of biological objects in tissue sections[2], generates reliable structural data for biological features in the mammalian brain[3]
This study documents a biological application of computerized stereology to estimate the total neuronal population in the frontal cortex of the vervet monkey brain (Chlorocebus aethiops sabeus), with assistance from two commercially available stereology programs, BioQuant Life Sciences and Stereologer (Figure 1)
Summary
Enter the slab sampling interval (if separate slabs of tissue were sliced exhaustively and each section is placed in sequential order enter 1 here). Enter the total number of sections taken through the reference space (in this case 760). Enter the section sampling interval (in this case 59). The system calculates the number of sections to be sampled (in this case 13)
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