Abstract
BackgroundThe present study has investigated the protein tyrosine phosphatase H1 (PTPH1) expression pattern in mouse brain and its impact on CNS functions.MethodsWe have previously described a PTPH1-KO mouse, generated by replacing the PTP catalytic and the PDZ domain with a LacZ neomycin cassette. PTPH1 expression pattern was evaluated by LacZ staining in the brain and PTPH1-KO and WT mice (n = 10 per gender per genotype) were also behaviorally tested for CNS functions.ResultsIn CNS, PTPH1 is expressed during development and in adulthood and mainly localized in hippocampus, thalamus, cortex and cerebellum neurons. The behavioral tests performed on the PTPH1-KO mice showed an impact on working memory in male mice and an impaired learning performance at rotarod in females.ConclusionThese results demonstrate for the first time a neuronal expression of PTPH1 and its functionality at the level of cognition.
Highlights
The present study has investigated the protein tyrosine phosphatase H1 (PTPH1) expression pattern in mouse brain and its impact on CNS functions
In the present study we focused our attention on a nerve crush lesion [8] and PTPMEG (NRPTP), PTPH1, and on its possible role on neural functions
LacZ staining in whole mount In PTPH1-KO adult animals, LacZ staining in the brain was observed in the cerebellum, hippocampus and in the thalamic nuclei
Summary
The present study has investigated the protein tyrosine phosphatase H1 (PTPH1) expression pattern in mouse brain and its impact on CNS functions. Tyrosine phosphorylation plays an important role in several signaling pathways regulating cell growth, differentiation, cell cycle, apoptosis and neuronal functions [1,2]. PTPs can be distinguished into four classes: 1) classical PTPs that can be subdivided into transmembrane, receptor-like enzymes, and the intracellular, nonreceptor PTPs, 2) dual-specificity PTPs (Ser and Tyr phosphatases), 3). Classical PTPs have been reported to play a key role in neural functions, from development to cognitive function. RPTPs such as PTPδ, PTPσ, LAR, and especially PTPRO, are important players in axonal growth and guidance during development [4]. Studies on PTPσKO (RPTP) mice have shown involvement of this PTP in the regulation of the developing hypothalamo-pituitary (page number not for citation purposes)
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