Knocking down TRPC1 expression by siRNA inhibits proliferation and invasiveness of human lung adenocarcinoma cell A549 in vitro

Abstract

To evaluate the effects of down-regulated expression of transient receptor potential canonical (TRPC1) by RNA interference (RNAi) on proliferation and invasiveness of human lung adenocarcinoma cell A549 in vitro. A549 cells were transfected with chemically synthesized small interfering RNA (siRNA) targeting TRPC1 gene. The mRNA and protein of TRPC1 were analyzed by real-time polymerase chain reaction (PCR) and Western blot respectively. To assess malignant phenotypes of transfected A549 cells, the assays of methyl thiazolyl tetrazolium (MTT), cell cycle and cell invasion were performed. siRNA targeting TRPC1 dramatically suppressed TRPC1 expression. In vitro study showed that siRNA targeting TRPC1 significantly inhibited cell proliferation of A549 cells with an inhibitory rate of 34.7% while negative control siRNA had no effect on cell proliferation. Flow cytometric analysis showed that siRNA targeting TRPC1 increased the number of cells in G0/G1 phase (P < 0.05) . Moreover, a knockdown of TRPC1 expression effectively inhibited cell invasiveness in A549 cells (P < 0.05) . Knocking down TRPC1 expression can inhibit proliferation and invasiveness of A549 cells in vitro.