Abstract
Background: Endometrial carcinoma (EC) still threatens the health of women. Thus, to explore how long intergenic non-protein coding RNA 01220 regulates the development of EC.Methods: Whole genome expression profile data of EC and paracancerous tissues in TCGA database were downloaded. LINC01220 expression in EC and paracancerous tissues of patients in our hospital were detected by qRT-PCR. Furthermore, the relationship between LINC01220 expression and clinicopathological features of EC patients was analyzed. After transfection with sh-LINC01220 and pcDNA-MAPK11 (mitogen-activated protein kinase) in EC cells, proliferative, colony formation abilities and apoptosis were determined by cell counting kit-8 (CCK-8), colony formation assay and flow cytometry, respectively. Western blot was conducted to determine the regulatory role of LINC01220 on MAPK11.Results: TCGA data showed that LINC01220 expression is markedly higher in EC tissues than that of paracancerous tissues, which was consistent without detection in EC patients of our hospital. LINC01220 expression was positively correlated to pathological grade and International Federation of Gynecology and Obstetrics (FIGO) stage of EC patients. After knockdown of LINC01220 in EC cells, proliferative and colony formation abilities decreased, whereas apoptotic rate increased. Cor function analysis revealed the positive correlation between LINC01220 and MAPK11 in EC. MAPK11 expression was regulated by LINC01220 in EC cells. Overexpression of MAPK11 can reverse the tumor suppressing effect of LINC01220 on EC.Conclusions: LINC01220 promotes EC development by stimulating proliferation and inhibiting apoptosis of EC cells through up-regulating MAPK11.
Highlights
Endometrial carcinoma (EC) still threatens the health of women
LINC01220 expression was markedly higher in EC tissues than that of paracancerous tissues, and the differential expression ratio was the highest (Figure 1B)
QRT-PCR was performed to detect the expression of LINC01220 in EC and paracancerous tissues of patients in our hospital
Summary
Endometrial carcinoma (EC) still threatens the health of women. to explore how long intergenic non-protein coding RNA 01220 regulates the development of EC. LINC01220 expression in EC and paracancerous tissues of patients in our hospital were detected by qRT-PCR. Results: TCGA data showed that LINC01220 expression is markedly higher in EC tissues than that of paracancerous tissues, which was consistent without detection in EC patients of our hospital. MAPK11 expression was regulated by LINC01220 in EC cells. Conclusions: LINC01220 promotes EC development by stimulating proliferation and inhibiting apoptosis of EC cells through up-regulating MAPK11. EC ranks fourth in female malignant tumors, second only to breast cancer, colorectal cancer and lung cancer. It ranks seventh in female tumors in developing countries [2]. Current treatments of EC mainly include hormone therapy, hysterectomy and combination therapy of chemotherapy and radiotherapy These measures are effective for early-stage EC. Exploring the molecular mechanisms, identifying new diagnostic and prognostic markers, and exploring new therapeutic strategies for EC are critical to improve the clinical outcomes
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