Abstract
BackgroundOvarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection. Ribosomal protein S6 (RPS6) is a ribosomal protein involved in the ribosomal 40S subunit, but its biological role in epithelial ovarian cancer (EOC) is still unknown.ResultsRPS6 was elevated in EOC compared to normal ovarian tissues and adenomas. Higher expression of RPS6 predicted worse prognosis in EOC. The level of RPS6 was correlated with clinical stage, histological type and pathological grade. Knockdown of RPS6 reduced the proliferation of ovarian cancer cell lines SKOV-3 and HO8910, and inhibit the migration and invasion ability. It revealed that cells arrested at G0G1 phase after knockdown of RPS6, and the expressions of CyclinD1, Cyclin E, CDK2, CDK4, CDK6 and pRb were also reduced.ConclusionsRPS6 is involved in EOC and knockdown of RPS6 could inhibit the proliferation, invasion and migration ability of EOC in vitro by inducing G0/G1 phase arrest. RPS6 is expected to be a novel biomarker and molecular target to the EOC.
Highlights
Ovarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection
Ribosomal protein S6 (RPS6) is overexpressed in epithelial ovarian cancer (EOC) tissues and its expression is associated with poor clinical outcomes RPS6 expression was examined by immunohistochemistry in a total of 252 tissue samples, of which 183 were EOC
There was no significant difference in RPS6 expression between serous and non-serous carcinomas (Fig. 1c, d; Table 1), nor between samples from individuals aged > or ≤ 50 years; RPS6 expression was significantly associated with FIGO stage (Fig. 1e-h), and pathological grade (Table 1)
Summary
Ovarian cancer typically is diagnosed late because insensitivity and lack of specificity of current biomarkers prior to its clinical detection. Ribosomal protein S6 (RPS6) is a ribosomal protein involved in the ribosomal 40S subunit, but its biological role in epithelial ovarian cancer (EOC) is still unknown. A major factor implicated in the high mortality rates from ovarian cancer is that the disease has frequently reached an advanced stage at primary diagnosis [3], as there are no specific clinical symptoms in the early stages. RPS6 is an important protein constituent of the 40S small subunit, located in the mRNA binding site of cytosolic ribosomes, and is the first ribosome protein discovered to undergo phosphorylation [9]. The specific function of RPS6 in the development of ovarian cancer remains unclear and warrants further investigation
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
