Abstract
Mediator complex (MED) contains 28 subunits, functions as a transcription machinery through interaction with RNA polymerase II and modulates gene expression involved in cell survival and growth. MED27, as an oncogene, stimulates malignant behavior of various tumors. Role of MED27 in gastric cancer was assessed in this study. Firstly, bioinformatics analysis predicted that MED27 was elevated in gastric cancer. Gastric cancer cells also showed higher expression of MED27 than normal gastric epithelial cells. Secondly, functional assays revealed that silencing of MED27 decreased cell viability, and reduced proliferation of gastric cancer. Cell invasion and migration of gastric cancer were also inhibited by loss of MED27. Moreover, knockdown of MED27 inhibited angiogenesis of gastric cancer. Thirdly, nuclear protein of β-catenin in gastric cancer was reduced by silencing of MED27. Lastly, in vivo tumor growth of gastric cancer was suppressed by interference of MED27. In conclusion, MED27 functioned as an oncogene in gastric cancer through promoting cell metastasis and angiogenesis.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
