Knockdown of LINC00662 represses AK4 and attenuates radioresistance of oral squamous cell carcinoma

Yangzong Chen,Xinshi Lin,Yimou Fu,Xiuxing Zhang,Chunchun Bao

doi:10.1186/s12935-020-01286-9

Abstract

BackgroundLncRNAs play crucial roles in the development of carcinomas. However, the investigation of LINC00662 in Oral squamous cell carcinoma (OSCC) is still elusive.MethodsqRT-PCR assay tested the expression levels of LINC00662, hnRNPC and AK4. With exposure to irradiation, CCK-8, colony formation, flow cytometry and western blot experiments, respectively determined the function of LINC00662 in the radiosensitivity of OSCC cells. Then RIP and western blot assays affirmed the interaction between hnRNPC protein and LINC00662 or AK4. Finally, rescue assays validated the regulation mechanism of LINC00662 in the radioresistance of OSCC.ResultsIn the present report, LINC00662 was overexpressed in OSCC and its silencing could alleviate radioresistance of OSCC. Furthermore, the interaction between hnRNPC protein and LINC00662 or AK4 was uncovered. Besides, LINC00662 regulated AK4 mRNA stability through binding to hnRNPC protein. To sum up, LINC00662 modulated the radiosensitivity of OSCC cells via hnRNPC-modulated AK4.ConclusionThe molecular mechanism of the LINC00662/hnRNPC/AK4 axis was elucidated in OSCC, which exhibited a promising therapeutic direction for patients with OSCC.

Highlights

LncRNAs play crucial roles in the development of carcinomas
LINC00662 silencing attenuated radioresistance of Oral squamous cell carcinoma (OSCC) cells To explore the expression profiles of LINC00662 in OSCC, we firstly tested its expression in OSCC cell lines (CAL27, SCC-4, SCC-1, TU183 and SCC-9) and the primary normal human oral keratinocytes (NHOK) cell lines. Quantitative real-time PCR (qRT-PCR) revealed that the expression of LINC00662 was relatively up-regulated in OSCC cells (Fig. 1a)
In order to investigate the effect of LINC00662 on the radioresistance of OSCC, shLINC00662#1 or sh-LINC00662#2 was transfected into CAL27 and SCC-4 cells

Summary

Introduction

LncRNAs play crucial roles in the development of carcinomas. The investigation of LINC00662 in Oral squamous cell carcinoma (OSCC) is still elusive. Oral squamous cell carcinoma (OSCC) is one of the most aggressive head and neck cancers all over the world [1]. A better understanding of the molecular regulation mechanism in OSCC was needed. Long non‐coding RNAs (lncRNAs), a sort of non‐coding RNAs (ncRNAs), have more than 200 nucleotides in length and play crucial roles in. Chen et al Cancer Cell Int (2020) 20:244 and cell invasion [11, 12]. LINC00662 boosts cell invasion and cancer stem cell-like phenotypes in lung cancer [13]. We aimed to investigate the role and regulation mechanism of LINC00662 in OSCC cell radioresistance

Objectives

Methods

Results

Discussion

Conclusion