Knockdown of circ_0067934 inhibits gastric cancer cell proliferation, migration and invasion via the miR‑1301‑3p/KIF23 axis.

Abstract

In recent years, circular RNAs (circRNAs/circs) have attracted significant attention due to their potentially important functions in a variety of human cancer types. circ_0067934 is a newly identified circRNA, the role of which in gastric cancer (GC) has yet to be reported, to the best of our knowledge. In the present study, the expression levels of circ_0067934, microRNA (miR)-1301-3p and kinesin family member 23 (KIF23) in GC cells were detected via reverse transcription-quantitative PCR. Cell proliferation was measured using Cell Counting Kit-8 assays and EdU staining. Wound healing and Transwell assays were performed to assess cell migration and invasion, respectively. Western blotting was performed to measure the protein expression levels of Ki67, proliferating cell nuclear antigen, MMP2, MMP9 and KIF23. The starBase database and luciferase reporter assays were used to predict and verify the binding between circ_0067934 and miR-1301-3p, as well as KIF23, in GC cells. The results demonstrated that circ_0067934 expression was upregulated in GC cells, and circ_0067934 silencing significantly inhibited GC cell proliferation, migration and invasion. In addition, miR-1301-3p was regulated by circ_0067934, and miR-1301-3p overexpression suppressed GC cell migration, invasion and proliferation. miR-1301-3p was found to target KIF23, and KIF23 overexpression reversed the effects of circ_0067934 silencing and miR-1301-3p overexpression on cell proliferation, migration and invasion. In conclusion, circ_0067934 may regulate the proliferation, invasion and migration of GC cells via the miR-1301-3p/KIF23 signaling axis, which may represent a novel therapeutic target for GC metastasis.