Abstract
Drought stress is a major environmental stress, which adversely affects the biological and molecular processes of plants, thereby impairing their growth and development. In the present study, we found that the expression level of OsTBP2.2 which encodes for a nucleus-localized protein member belonging to transcription factor IID (TFIID) family, was significantly induced by polyethylene glycol (PEG) treatment. Therefore, knockdown mutants of OsTBP2.2 gene were generated to investigate the role of OsTBP2.2 in rice response to drought stress. Under the condition of drought stress, the photosynthetic rate, transpiration rate, water use efficiency, and stomatal conductance were significantly reduced in ostbp2.2 lines compared with wild type, Dongjin (WT-DJ). Furthermore, the RNA-seq results showed that several main pathways involved in “MAPK (mitogen-activated protein kinase) signaling pathway”, “phenylpropanoid biosynthesis”, “defense response” and “ADP (adenosine diphosphate) binding” were altered significantly in ostbp2.2. We also found that OsPIP2;6, OsPAO and OsRCCR1 genes were down-regulated in ostbp2.2 compared with WT-DJ, which may be one of the reasons that inhibit photosynthesis. Our findings suggest that OsTBP2.2 may play a key role in rice growth and the regulation of photosynthesis under drought stress and it may possess high potential usefulness in molecular breeding of drought-tolerant rice.
Highlights
Abiotic stress has a significant inhibitory effect on plant growth and development, and even affects crop productivity in severe cases [1,2,3,4,5,6]
In order to determine spatial expression pattern of OsTBP2.2 gene intested, rice, we performed qRTshowed that OsTBP2.2 gene wastheconstitutively expressed in all organs its expression
Showed that OsTBP2.2 gene was constitutively expressed in all organs tested, its expression higher, respectively
Summary
Abiotic stress has a significant inhibitory effect on plant growth and development, and even affects crop productivity in severe cases [1,2,3,4,5,6]. Drought stress is the most prevalent environmental threat in the agricultural sector, affecting approximately 75% of the global harvested areas and leading to a significant decline in crop yield worldwide [7]. Photosynthesis is one of the essential plant biological processes which is sensitive to water deficit. Photosynthetic metabolism was severely inhibited, including photophosphorylation [8], ATP (adenosine triphosphate) synthesis [9], and subsequently lead to significant decline in the photosynthetic rate [10]. In the absence of water, stomata are closed to reduce
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
