Abstract
B cell-specific moloney murine leukemia virus integration site 1 (BMI1) is a transcriptional repressor of polycomb repressive complex 1, which is involved in the proliferation, senescence, migration, and tumorigenesis of cancer. Experimental researchers have convincingly linked BMI1 to tumorigenesis. However, there is no study about the issue on the role of BMI1 in the proliferation, apoptosis, and migration of bladder cancer. To address this question, we examined the expression of BMI1 in bladder cancer tissues and used siRNA to knockdown BMI1 expression in bladder cancer T24 cells. Then we tested the cell proliferation by CCK8 assay and soft agar colony formation assay, apoptosis by flow cytometry assay, and cell invasiveness by transwell migration assay. Our results revealed that BMI1 promoted proliferation, migration, invasion, and progression in bladder cancer. Over-expression of BMI1 was correlated with tumor clinic-pathological features. BMI1 siRNA effectively inhibited bladder cancer cell proliferation and migration in vitro, and it promoted bladder cancer invasion, maybe by causing epithelial-to-mesenchymal transition. Our findings suggested that BMI1 may represent a novel diagnostic marker and a therapeutic target for bladder cancer, and deserves further investigation.Electronic supplementary materialThe online version of this article (doi:10.1007/s11010-013-1745-0) contains supplementary material, which is available to authorized users.
Highlights
Bladder cancer is a frequent urological malignancy cancer and accounts for about 3 % of all cancer related deaths
We found that BMI1 was highly expressed in bladder cancer and its expression was correlated with clinic-pathological features
We demonstrated here that BMI1 promoted bladder cancer cell proliferation, migration, and progression by inhibition p16 and p14 expression
Summary
Bladder cancer is a frequent urological malignancy cancer and accounts for about 3 % of all cancer related deaths. In spite of recent progress in its diagnosis and treatment, the molecular mechanisms underlying the development and progression of bladder cancer remain poorly understood, and prognosis of invasive bladder cancer still remains unsatisfactory [1]. It is of great urgent to further investigate the molecular mechanisms of bladder cancer metastasis and progression, which will improve the prognosis of invasive bladder cancer. B-cell-specific moloney murine leukemia virus integration site 1 (BMI1) is a transcriptional repressor of polycomb repressive complex 1(PRC1), which is located at chromosome 10p11.23. It plays an essential role in embryogenesis and maintenance adult stem cell’s selfrenewal [2, 3]. High expression of BMI1 was associated with aggressive tumor behavior and poor outcome [10]
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